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KMID : 0380020030180040249
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 4 p.249 ~ p.254
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Two-Stage Fed-Batch Culture of Candida magnoliae for the Production of Erythritol using an Industrial Medium
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Park Sun-Young
Seo Jin-Ho
Ryu Yeon-Woo
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Abstract
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Experiments were carried out to select an industrial nitrogen source and optimize erythritol production by Candida magnoliae in fed-batch culture. Among the industrial nitrogen sources tested, light steep water (LSW) was found to be the best nitrogen source for producing erythritol, based on erythritol yield and raw material price. The maximum erythritol concentration obtained a 131.6 g/L, with a 52.6％ yield and 0.52 g/L-hr productivity from a 250 g/L glucose and 43.3 mL/L LSW in batch culture. Two-stage fed-batch culture was chosen to improve the volumetric productivity and the yield of erythritol. High cell density culture in cell growth stage was achieved by batch type culture containing 100 g/L glucose and 500 mL/L LSW. The cell concentration was 71.0 g/L after 23 hours of culture. Erythritol productivity was decreased by increasing glucose concentration in the production stage. But 37.3％ of the maximum erythritol yield was obtained with 185.5 g/L of erythritol and 1.66 g/L-hr of productivity when 820 g of glucose powder was directly added to a concentration of 450 g/L glucose in production stage.
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KEYWORD
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Light steep water, two-stage fed-batch culture, Candida magnoliae, erythritol
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